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robosep cd4 t-cell isolation kit  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc robosep cd4 t-cell isolation kit
    Pancreas-infiltrating macrophages from superoxide-deficient NOD.Rag mice display a decrease in TNF-α and IL-1β synthesis, and a concomitant increase in Arg-1 synthesis after diabetogenic <t>CD4</t> T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1– expressing (B) macrophages in the pancreata of NOD.Rag and NOD.Rag.Ncf1m1J recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 were calculated from CD11b+, I-Ag7+ cells. Data are representative of four independent experiments.
    Robosep Cd4 T Cell Isolation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/robosep+cd4+t-cell+isolation+kit/robosep+kit+++17951++human+t+cell+isolation+kit+/pmc04338593-62-24-29
    Average 90 stars, based on 1 article reviews
    robosep cd4 t-cell isolation kit - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes"

    Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes

    Journal: Diabetes

    doi: 10.2337/db14-0929

    Pancreas-infiltrating macrophages from superoxide-deficient NOD.Rag mice display a decrease in TNF-α and IL-1β synthesis, and a concomitant increase in Arg-1 synthesis after diabetogenic CD4 T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1– expressing (B) macrophages in the pancreata of NOD.Rag and NOD.Rag.Ncf1m1J recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 were calculated from CD11b+, I-Ag7+ cells. Data are representative of four independent experiments.
    Figure Legend Snippet: Pancreas-infiltrating macrophages from superoxide-deficient NOD.Rag mice display a decrease in TNF-α and IL-1β synthesis, and a concomitant increase in Arg-1 synthesis after diabetogenic CD4 T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1– expressing (B) macrophages in the pancreata of NOD.Rag and NOD.Rag.Ncf1m1J recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 were calculated from CD11b+, I-Ag7+ cells. Data are representative of four independent experiments.

    Techniques Used: Expressing, Generated, Fluorescence

    Pancreas-infiltrating macrophages from NOD.Rag mice treated with MnP display a decrease in TNF-α and a concomitant increase in Arg-1 synthesis after diabetogenic CD4 T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1–expressing (B) macrophages in the pancreata of NOD.Rag recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells treated with HBSS or MnP. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 mean fluorescence intensity were calculated from CD11b+, I-Ag7+ cells. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells, while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Data are representative of four independent experiments.
    Figure Legend Snippet: Pancreas-infiltrating macrophages from NOD.Rag mice treated with MnP display a decrease in TNF-α and a concomitant increase in Arg-1 synthesis after diabetogenic CD4 T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1–expressing (B) macrophages in the pancreata of NOD.Rag recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells treated with HBSS or MnP. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 mean fluorescence intensity were calculated from CD11b+, I-Ag7+ cells. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells, while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Data are representative of four independent experiments.

    Techniques Used: Expressing, Generated, Fluorescence

    Related Articles

    Isolation:

    Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes
    Article Snippet: CD4 T cells were purified by negative selection according to the manufacturer’s instructions via the RoboSep CD4 T-cell isolation kit (STEMCELL Technologies) with >90% purity ( 17 ).

    Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes
    Article Snippet: CD4 T-Cell Isolation, Expansion, and Adoptive Transfer of T1D CD4 T cells were purified by negative selection according to the manufacturer’s instructions via the RoboSep CD4 T-cell isolation kit (STEMCELL Technologies) with >90% purity ( 17 ).

    Adoptive Transfer Assay:

    Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes
    Article Snippet: CD4 T cells were purified by negative selection according to the manufacturer’s instructions via the RoboSep CD4 T-cell isolation kit (STEMCELL Technologies) with >90% purity ( 17 ).

    Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes
    Article Snippet: CD4 T-Cell Isolation, Expansion, and Adoptive Transfer of T1D CD4 T cells were purified by negative selection according to the manufacturer’s instructions via the RoboSep CD4 T-cell isolation kit (STEMCELL Technologies) with >90% purity ( 17 ).

    Purification:

    Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes
    Article Snippet: CD4 T cells were purified by negative selection according to the manufacturer’s instructions via the RoboSep CD4 T-cell isolation kit (STEMCELL Technologies) with >90% purity ( 17 ).

    Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes
    Article Snippet: CD4 T-Cell Isolation, Expansion, and Adoptive Transfer of T1D CD4 T cells were purified by negative selection according to the manufacturer’s instructions via the RoboSep CD4 T-cell isolation kit (STEMCELL Technologies) with >90% purity ( 17 ).

    Selection:

    Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes
    Article Snippet: CD4 T cells were purified by negative selection according to the manufacturer’s instructions via the RoboSep CD4 T-cell isolation kit (STEMCELL Technologies) with >90% purity ( 17 ).

    Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes
    Article Snippet: CD4 T-Cell Isolation, Expansion, and Adoptive Transfer of T1D CD4 T cells were purified by negative selection according to the manufacturer’s instructions via the RoboSep CD4 T-cell isolation kit (STEMCELL Technologies) with >90% purity ( 17 ).

    Expressing:

    Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes
    Article Snippet: CD4 T cells were purified by negative selection according to the manufacturer’s instructions via the RoboSep CD4 T-cell isolation kit (STEMCELL Technologies) with >90% purity ( 17 ).

    Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes
    Article Snippet: CD4 T-Cell Isolation, Expansion, and Adoptive Transfer of T1D CD4 T cells were purified by negative selection according to the manufacturer’s instructions via the RoboSep CD4 T-cell isolation kit (STEMCELL Technologies) with >90% purity ( 17 ).

    Generated:

    Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes
    Article Snippet: CD4 T cells were purified by negative selection according to the manufacturer’s instructions via the RoboSep CD4 T-cell isolation kit (STEMCELL Technologies) with >90% purity ( 17 ).

    Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes
    Article Snippet: CD4 T-Cell Isolation, Expansion, and Adoptive Transfer of T1D CD4 T cells were purified by negative selection according to the manufacturer’s instructions via the RoboSep CD4 T-cell isolation kit (STEMCELL Technologies) with >90% purity ( 17 ).

    Fluorescence:

    Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes
    Article Snippet: CD4 T cells were purified by negative selection according to the manufacturer’s instructions via the RoboSep CD4 T-cell isolation kit (STEMCELL Technologies) with >90% purity ( 17 ).

    Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes
    Article Snippet: CD4 T-Cell Isolation, Expansion, and Adoptive Transfer of T1D CD4 T cells were purified by negative selection according to the manufacturer’s instructions via the RoboSep CD4 T-cell isolation kit (STEMCELL Technologies) with >90% purity ( 17 ).



    Similar Products

    robosep cd4 t-cell isolation kit  (STEMCELL Technologies Inc)
    90
    STEMCELL Technologies Inc robosep cd4 t-cell isolation kit
    Pancreas-infiltrating macrophages from superoxide-deficient NOD.Rag mice display a decrease in TNF-α and IL-1β synthesis, and a concomitant increase in Arg-1 synthesis after diabetogenic <t>CD4</t> T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1– expressing (B) macrophages in the pancreata of NOD.Rag and NOD.Rag.Ncf1m1J recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 were calculated from CD11b+, I-Ag7+ cells. Data are representative of four independent experiments.
    Robosep Cd4 T Cell Isolation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/robosep+cd4+t-cell+isolation+kit/robosep+kit+++17951++human+t+cell+isolation+kit+/pmc04338593-62-24-29
    Average 90 stars, based on 1 article reviews
    robosep cd4 t-cell isolation kit - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Pancreas-infiltrating macrophages from superoxide-deficient NOD.Rag mice display a decrease in TNF-α and IL-1β synthesis, and a concomitant increase in Arg-1 synthesis after diabetogenic CD4 T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1– expressing (B) macrophages in the pancreata of NOD.Rag and NOD.Rag.Ncf1m1J recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 were calculated from CD11b+, I-Ag7+ cells. Data are representative of four independent experiments.

    Journal: Diabetes

    Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes

    doi: 10.2337/db14-0929

    Figure Lengend Snippet: Pancreas-infiltrating macrophages from superoxide-deficient NOD.Rag mice display a decrease in TNF-α and IL-1β synthesis, and a concomitant increase in Arg-1 synthesis after diabetogenic CD4 T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1– expressing (B) macrophages in the pancreata of NOD.Rag and NOD.Rag.Ncf1m1J recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 were calculated from CD11b+, I-Ag7+ cells. Data are representative of four independent experiments.

    Article Snippet: CD4 T-Cell Isolation, Expansion, and Adoptive Transfer of T1D CD4 T cells were purified by negative selection according to the manufacturer’s instructions via the RoboSep CD4 T-cell isolation kit (STEMCELL Technologies) with >90% purity ( 17 ).

    Techniques: Expressing, Generated, Fluorescence

    Pancreas-infiltrating macrophages from NOD.Rag mice treated with MnP display a decrease in TNF-α and a concomitant increase in Arg-1 synthesis after diabetogenic CD4 T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1–expressing (B) macrophages in the pancreata of NOD.Rag recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells treated with HBSS or MnP. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 mean fluorescence intensity were calculated from CD11b+, I-Ag7+ cells. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells, while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Data are representative of four independent experiments.

    Journal: Diabetes

    Article Title: Loss of NADPH Oxidase–Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes

    doi: 10.2337/db14-0929

    Figure Lengend Snippet: Pancreas-infiltrating macrophages from NOD.Rag mice treated with MnP display a decrease in TNF-α and a concomitant increase in Arg-1 synthesis after diabetogenic CD4 T-cell transfer. Dot plots of TNF-α– (A), IL-1β–, and Arg-1–expressing (B) macrophages in the pancreata of NOD.Rag recipient mice at 7 days post-transfer with NOD.BDC-2.5 CD4 T cells treated with HBSS or MnP. Dot plots were generated by first gating on granulocytes and then gating on F4/80+ or CD11b+ cells via side scatter. Mean fluorescence intensity (MFI) values of TNF-α and IL-1β were quantified by F4/80+, I-Ag7+ cells, while those of Arg-1 mean fluorescence intensity were calculated from CD11b+, I-Ag7+ cells. For TNF-α and IL-1β, expression is shown by F4/80+, I-Ag7+ cells, while Arg-1 expression by CD11b+, I-Ag7+ cells is displayed. Data are representative of four independent experiments.

    Article Snippet: CD4 T-Cell Isolation, Expansion, and Adoptive Transfer of T1D CD4 T cells were purified by negative selection according to the manufacturer’s instructions via the RoboSep CD4 T-cell isolation kit (STEMCELL Technologies) with >90% purity ( 17 ).

    Techniques: Expressing, Generated, Fluorescence